Molecular-genetic characterisation of hepatitis B virus detected for the first time in blood donors
Abstract
Objective. To determine the prevalence of markers of parenteral viral hepatitis B in donors of blood and its components in the Republic of Belarus.
Materials and methods. The study included 61 blood serum/plasma samples collected from donors from all regions of the country. All samples were examined by CLIA/ELISA and PCR for the presence of hepatitis B virus markers.
Results. As studies have shown, out of 61 sequenced samples, 58 (95.1 %) belonged to genotype D, and 3 (4.9 %) to A. Genotype D in 47 (81.1 %) cases was represented by the D2 subgenotype, in 5 (8.6 %) samples, the D1 subgenotype was detected, in 5 (8.6 %) — D3 and in 1 (1.7 %) sample the D4 subgenotype was detected, all samples of genotype A belonged to the A2 subgenotype. All DNA sequences of the D1 and D3 subgenotypes were assigned to the ayw2 subtype, D4 — to the ayw3 subtype, most samples of the D2 subgenotype were of the ayw3 subtype, and one sample each was assigned to the ayw2 and ayw4 subtypes. All DNA sequences of the A2 subgenotype belonged to the adw2 subtype.
Conclusion. The HBV variants identified in donors during phylogenetic analysis were in the same cluster with previously described sequences from Belarus, which indicates that the epidemiological process of hepatitis B in the country is supported by “local domestic” viruses.
About the Authors
F. N. Karpenko
Республиканский научно-практический центр трансфузиологии и медицинских биотехнологий
Belarus
Belarus
V. F. Eremin
Республиканский научно-практический центр трансфузиологии и медицинских биотехнологий
Belarus
Belarus
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For citations:
Karpenko F.N., Eremin V.F. Molecular-genetic characterisation of hepatitis B virus detected for the first time in blood donors. Healthcare. 2024;(12):54-65. (In Russ.)
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